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| Registros recuperados: 27 | |
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| Pérez de la Torre,Mariana C.; Escandón,Alejandro S.. |
| The objective of the present work was to establish the molecular identification profile for six new varieties of Nierembergia linariaefolia to incorporate the fingerprint, as complementary information to the standard registration data. Total DNA was extracted from young leaves following the protocol of the cetyltrimethylammonium bromide. Anchored microsatellites were used as molecular markers. The amplification reactions were carried out with seven primers. A total of 251 loci were detected, 98% of them were polymorphic. The average of polymorphic loci was 35 loci per primer and, 41 loci per genotype. Six out of the seven primers used discriminated all the individuals involved in the present study; consequently, it was possible to generate the molecular... |
| Tipo: Journal article |
Palavras-chave: ISSR; Ornamentals; Varietal identification. |
| Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300014 |
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| Saleh,Basel. |
| The goal of this study was to investigate the molecular characterization of A. macrostachyum genotypes grown in the Western coast of Syria using RAPD and ISSR techniques. PCR amplifications with 20 RAPD primers gave an average of 9.25 selected markers/ primer, with a maximum of 17 (OPG05) and a minimum of four (OPA02). Percentage of polymorphic bands ranged from 40 to 100% according to RAPD primers tested. Among the 185 selected bands, 160 (84.96%) were polymorphic. The amplification with seven ISSR primers generated 88 bands, and 80 (90.91%) were polymorphic. (CA)8RG and (AG)8GTG ISSR primers tested in this study yielded highly informative patterns. RAPD and ISSR fragment sizes ranged from 0.2-3 kb. Based on this study, the use of RAPD and ISSR... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: A. macrostachyum; Genetic diversity; Genotype; ISSR; Polymorphism; RAPD. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500002 |
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| Chagas,Kyvia Pontes Teixeira das; Freire,Ageu da Silva Monteiro; Pinheiro,Luciana Gomes; Fajardo,Cristiane Gouvêa; Vieira,Fábio de Almeida. |
| Abstract This study aimed to estimate the genetic diversity of three Elaeis guineensis populations in Rio Grande do Norte state, as well as to verify the decreases in effective population size. The population with the highest polymorphism was MAT (Mata) with+ 57 loci (72%), followed by RIA (Riacho) with 54 loci (68%) and HOR (Horta) with 34 loci (43.03%). The RIA population was shown to be the most genetically diverse, with Nei (h = 0.28) and Shannon (I = 0.41) diversity indices. There was high genetic differentiation among populations (AMOVA, analysis of molecular variance = 42%), which was separated into three distinct genetic groups according to a Bayesian analysis. There was a significant population decrease (P < 0.05) for the HOR population in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular Marker; Bottleneck; ISSR; Conservation. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000400801 |
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| Paschoa,Roberta Pena da; Christ,Jheniffer Abeldt; Valente,Cecília Silva; Ferreira,Marcia Flores da Silva; Miranda,Fábio Demolinari de; Garbin,Mário Luís; Carrijo,Tatiana Tavares. |
| ABSTRACT Although a species’ sexual system may influence the genetic diversity of its populations in their natural environment, there have been few such studies involving indigenous species of the Atlantic Forest. Here we study Myrsine coriacea, a dioecious tree widely used in reforestation programs despite a lack of information about its natural interpopulation genetic variation. To address this knowledge gap, intra- and interpopulation genetic diversity were measured for male and female individuals of ten natural populations using ISSR markers. Greater intrapopulation genetic diversity indicated interpopulation gene flow, regardless of isolation and distance between populations. Multivariate analyses detected significant differences in genetic diversity... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Capororoca; Conservation; ISSR; Myrsinaceae; Rapanea. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000300376 |
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| Al- Safadi,Bassam; Faouri,Hussam; Elias,Rana. |
| This work investigated the genetic diversity and relationships among Capparis species growing in Syria using IRAP and ISSR techniques. Forty-seven samples of three Capparis species genotypes were collected from 21 different locations in Syria. The genotypes were morphologically identified based on the descriptions available in the literature. When IRAP technique was used, an average of 71.5% of the amplified fragments were polymorphic compared to 82.04% in ISSR. Morphological characterization along with the cluster and PCoA analyses of the data divided the studied genotypes into three groups. The groups included genotypes identified as Capparis spinosa L, C. sicula Duh., and C. aegyptia Lam. Based on the morphological description, molecular studies and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Capparis; Diversity; ISSR; IRAP; Syria. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000600916 |
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| Jesus,Izabela Santos Dias de; Conceição,Leila Patricio; Schnadelbach,Alessandra Selbach; Assis,José Geraldo de Aquino; Carvalho,Maria Luiza Silveira de. |
| ABSTRACT Discocactus zehntneri subsp. petr-halfari, an endangered taxon, is represented by a single population in an anthropized area of Bahia, Brazil, where it is suffering due to extreme extractivism. Thus, information about this cactus, such as its reproductive patterns, is urgently needed to support conservation strategies. A population genetics approach was used to determine if this subspecies has a preferential pattern of reproduction. We sampled 18 individuals, both with and without connection to parental plants, from five clumps and assessed their diversity and genetic structure using five ISSR markers. The results revealed two clumps that are genetically supported by the presence of genetically equal individuals. The other three groups presented... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Discocactus; ISSR; Genetic variabilty; Microendemism; Reproductive strategies. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000300437 |
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| Hu,Yanping; Xie,Xiaolong; Wang,Li; Zhang,Huaigang; Yang,Jian; Li,Yi. |
| To examine whether cultivation reduced genetic variation in the important Chinese medicinal plant Rheum tanguticum, the levels and distribution of genetic variation were investigated using ISSR markers. Fifty-eight R. tanguticum individuals from five cultivated populations were studied. Thirteen primers were used and a total of 320 DNA bands were scored. High levels of genetic diversity were detected in cultivated R. tanguticum (PPB = 82.19, H = 0.2498, H B = 0.3231, I = 0.3812) and could be explained by the outcrossing system, as well as long-lived and human-mediated seed exchanges. Analysis of molecular variance (AMOVA) showed that more genetic variation was found within populations (76.1%) than among them (23.9%). This was supported by the coefficient... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cultivated populations; Genetic variation; ISSR; Polygonaceae; Rheum tanguticum Maxim. ex Balf. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400010 |
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| Chen,Liyuan; Chen,Faju; He,Suichao; Ma,Luyi. |
| Background Genetic diversity and genetic variation of 10 populations and subpopulations of Magnolia wufengensis, a new and endangered endemic species, were examined by inter simple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) molecular markers. Compared with other endangered endemic Magnolia taxa, M. wufengensis holds a relatively high level of genetic variation. Result Total genetic diversity was found to be 87.7% for ISSR and 88.0% for SRAP markers. For polymorphic loci (P), the effective mean number of alleles (Ae) was 1.414 for ISSR markers and 1.458 for SRAP markers, while the mean expected heterozygosity (H) was 0.256 using ISSR and 0.291 for SRAP markers. Within-population variation was estimated for P as 74.9% using... |
| Tipo: Journal article |
Palavras-chave: Genetic structure; ISSR; Population genetic diversity; SRAP. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600003 |
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| Hakki,Erdogan E; Kayis,Seyit A; Pinarkara,Emine; Sag,Ayla. |
| Cannabis sativa L. is a multiple-use plant that provides raw material for the production of seed oil, natural fiber for textiles, automotive and pulp industries. It has also been used in insulating boards, ropes, varnishes, animal feed, and as medicinal agents. Cannabis has potential to be used for phytoremediation: however, its cultivation is strictly controlled due to its psychoactive nature and usage in producing drugs such as marijuana, and hashish. In this study, psychoactive type Cannabis samples, which were seized from 23 different locations of Turkey, and nine hemp type Cannabis accessions, as well as an unknown accession were used. Our interest was to identify the genetic relatedness of the seized samples and to separate drug and hemp type plants.... |
| Tipo: Journal article |
Palavras-chave: Cluster analysis; Hemp; ISSR; Marijuana; Principle coordinate analysis. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400010 |
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| Registros recuperados: 27 | |
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